The next 28th and 29th of October we have a date. Join us at the 2021 ISEV Workshop: massivEVs – an ISEV workshop on massive production of EVs co-organized between VES4US and EVFOUNDRY. The event will take place on Brescia, Italy in the Hotel Acquaviva del Garda.

The goal of the workshop is to address and channel the efforts towards large scale EV production and manufacturing. Themes ranging from the underpinning science and technology of equipment and processes to good manufacturing practices (GMPs) and requirements will be featured. It will feature a dialogue between an outstanding and diverse group of academic research scientists, clinicians and industrial partners involved in massive production of EVs.

Introductory invited talks, short talks selected from abstracts, posters and roundtable discussions will revolve around four highly interrelated themes related to large-scale EV production:

  • Massive production from Human sources and applications (including therapeutics, nutraceutics, cosmetics, EV based nanotechnology)
  • Massive production from other (than human) sources and applications
  • Upstream and downstream technologies and process upscaling
  • Validation, standardization, and regulatory issues.

The workshop is expected to lead to one or more products, such as an ISEV position paper, perspectives papers on massive EV production, etc. 50 participants will be selected based on their expertise. Here for more information about the workshop and the COVID measures

Agenda MassivEvs:

27th October 2021

18:00-19:00 Registration

19:00 Welcome reception

28th October 2021

8:00-8:30 Registration

8:30-8:40 Welcome (Paolo Bergese, Antonella Bongiovanni)

8:40-8:50 Overview of ISEV-MassivEVs workshop and products (Kenneth Witwer)

8:50-9:20 Opening talk – Marca Wauben, Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands. “Towards a robust characterization of EV-enriched massive preparations”

9:20-11:10 Topic 1 – Massive production from Human sources and applications (including therapeutics, nutraceutics, cosmetics, EV based nanotechnology)

Chairs: Annalisa Radeghieri and Marca Wauben

Plenary sessions (20’+5’)

Mario Gimona, Paracelsus Medical University, Salzburg, Austria. “Scalable manufacturing and quality control of naive human umbilical cord MSC-sEVs for clinical testing: Sufficient for the day is the evil thereof”

Eva Rohde, University Hospital Salzburger Landeskliniken GesmbH and Paracelsus Medical University, Salzburg, Austria. “Bridging the Translational Gap in Clinical EV-Tx Development”

Oral presentations (12’+3’)

  • 4 selected abstracts

11:10-11:30 Networking coffee break

11:30-13:10 Topic 3 – Upstream and downstream technologies and process upscalingChairs: Antonella Bongiovanni and Paolo Bergese

Plenary sessions (20’+5’)

Konstantin Konstantinov, Codiak, Cambridge, MA, USA. “Industrialization of the GMP Manufacture of Exosome Therapeutics and Opportunities for Further Multifold Process Productivity Increase”

Michiel Stork, Institute for Translational Vaccinology (Intravacc), Bilthoven, The Netherlands.  “Production of Outer Membrane Vesicles for vaccine purposes”

Oral presentations (12’+3’)

  • 3 selected abstracts

13:10-14:10 Networking lunch break

14:10-15:30 Parallel round table discussions (participants divided in 2 groups):

  • Round table on Topic 1 – First session (group 1), Moderators: chairs (Annalisa Radeghieri and Marca Wauben) and experts (Mario Gimona and Eva Rohde)
  • Round table on Topic 3 – First session (group 2), Moderators: chairs (Antonella Bongiovanni and Paolo Bergese) and experts (Konstantin Konstantinov and Michiel Stork)

15:30-16:00: Networking coffee break with posters

16:00-17:20: Parallel round table discussions (2 groups divided over 2 topics):

  • Round table on Topic 1 – Second session (group 2) Moderators: chairs (Annalisa Radeghieri and Marca Wauben) and experts (Mario Gimona and Eva Rohde)
  • Round table on Topic 3 – Second session (group 1) Moderators: chairs (Antonella Bongiovanni and Paolo Bergese) and experts (Konstantin Konstantinov and Michiel Stork)

17:20-18:00 Networking coffee break with posters

18:00-19:30 Wrap up round table Topic 1 and 3 + decision on possible workshop product(s)

19:30-20:00 Networking reception with posters

20:30 – Dinner on site

29th October 2021

9:00-10:40 Topic 2 – Massive production from other (than human) sources and applications

Chairs: Nicolas Touzet and Peter Nejsum

Plenary sessions (20’+5’)

Maria Kaparakis-Liaskos (La Trobe University, Melbourne, Australia) “Large scale production and purification of bacterial membrane vesicles”

Nicole Meisner-Kober (University of Salzburg, Salzburg, Austria) “Process development and industrial scale isolation of food-derived EVs”

Oral presentations (12’+3’)

  • 3 selected abstracts

10:40-11:00: Networking coffee break

11:00- 12:40 Topic 4 – Validation, standardization and regulatory issues

Chairs:  Eva Rohde and Kenneth Witwer

Plenary sessions (20’+5’)

Lang Tran (Institute of Occupational Medicine, Edinburgh, UK). “Biomaterials – Safe and Smart materials for Advanced Therapy. A Case Study – Treatment for Alzheimer’s Disease”

Sai Kiang Lim (Institute of Molecular and Cell Biology; Agency for Science, Technology and Research, Singapore). “Towards validation and standardized characterization of immortalized MSCs for massive MSC-EV production”

Oral presentations (12’+3’)

  • 3 selected abstracts

12:40-13:40 Networking lunch break

13:40-14:20 Parallel round table discussions (participants divided in 2 groups):

  • Round table on Topic 2 – First session (group 1), Moderators: chairs (Nicolas Touzet and Peter Nejsum) and experts (Maria Kaparakis-Liaskos and Nicole Meisner-Kober)
  • Round table on Topic 4 – First session (group 2), Moderators: chairs (Eva Rohde and Kenneth Witwer) and experts (to be confirmed)

14:20-14:50: Networking coffee break with posters

14:50-16:30 Parallel round table discussions (2 groups divided over 2 topics):

  • Round table on Topic 2 – Second session (group 2), Moderators: chairs (Nicolas Touzet and Peter Nejsum) and experts (Maria Kaparakis-Liaskos and Nicole Meisner-Kober)
  • Round table on Topic 4 – Second session (group 1), Moderators: chairs (Eva Rohde and Kenneth Witwer) and experts (to be confirmed)

16:30-17:30 Networking coffee break with posters

17:30-19:00 Wrap up round table Topic 2 and 4 + decision on possible workshop product(s)

19:00 Closing drink

20:30 Dinner in Desenzano del Garda (optional)